Evaluation of hha and hha sepB mutant strains of Escherichia coli O157:H7 as bacterins for reducing E. coli O157:H7 shedding in cattle
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چکیده
منابع مشابه
Evaluation of hha and hha sepB mutant strains of Escherichia coli O157:H7 as bacterins for reducing E. coli O157:H7 shedding in cattle.
Escherichia coli O157:H7 colonizes cattle intestines by using the locus of enterocyte effacement (LEE)-encoded proteins. The induction of systemic immune response against LEE-encoded proteins, therefore, will prove effective in reducing E. coli O157:H7 colonization in cattle. The previous studies have demonstrated that a hha (encodes for a hemolysin expression modulating protein) deletion enhan...
متن کاملComparison of two methods for detection of E. coli O157H7 in unpasteurized milk
BACKGROUND AND OBJECTIVES The most common serotype of enterohaemorrhagic Esherichia coli group or Shiga-toxin-producing E. coli is O157:H7. Domestic and wild ruminants are regarded as the main natural reservoirs. O157:H7 serotype is the major cause of gastrointestinal infections in developed countries. In this study was conducted to survey on the toxigenic E. coli O157: H7 strains in milk of in...
متن کاملEngineering global regulator Hha of Escherichia coli to control biofilm dispersal
The global transcriptional regulator Hha of Escherichia coli controls biofilm formation and virulence. Previously, we showed that Hha decreases initial biofilm formation; here, we engineered Hha for two goals: to increase biofilm dispersal and to reduce biofilm formation. Using random mutagenesis, Hha variant Hha13D6 (D22V, L40R, V42I and D48A) was obtained that causes nearly complete biofilm d...
متن کاملHha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility.
Escherichia coli Hha is an environmental-response regulator of the pathogenic hemolysin operon, and Hha and the contiguous YbaJ are both induced 30-fold in E. coli biofilms (Appl. Microbiol. Biotechnol. 64:515, 2004). Here it is shown that Hha and YbaJ regulate biofilm formation since the hha/ybaJ deletion reduces biofilm mass in microtitre plates (81% in minimal medium, 50% in complex medium) ...
متن کاملcomparison of two methods fordetection of e. coli o157h7 in unpasteurized milk
background and objectives: the most common serotype of enterohaemorrhagic esherichia coli group or shiga-toxin-producing e. coli is o157:h7. domestic and wild ruminants are regarded as the main natural reservoirs. o157:h7 serotype is the major cause of gastrointestinal infections in developed countries. in this study was conducted to survey on the toxigenic e. coli o157: h7 strains in milk of i...
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ژورنال
عنوان ژورنال: Vaccine
سال: 2011
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2011.04.073